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KMID : 0191120130280050672
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Journal of Korean Medical Science
2013 Volume.28 No. 5 p.672 ~ p.679
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Improvement of the Diagnostic Sensitivity of Scrub Typhus Using a Mixture of Recombinant Antigens Derived from Orientia tsutsugamushi Serotypes
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Kim Young-Jin
Yeo Seon-Ju
Park Sang-Jin
Woo Ye-Ju
Kim Min-Woo
Kim Seung-Han
Chang In-Ae
Jeon Sung-Ho
Park Byung-Jae
Song Gi-Jun
Lee Myoung-Gu
Kim Ik-Sang
Kim Yoon-Won
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Abstract
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Diagnosis of scrub typhus is difficult because its symptoms are very similar to other acute febrile illnesses, such as leptospirosis, murine typhus, and other viral hemorrhagic fevers. To differentiate scrub typhus from other acute febrile diseases, a rapid and reliable serological diagnosis is important. We have developed a chimeric recombinant antigen cr56 and two other recombinant antigens, r21 and kr56, from various serotypes of Orientia tsutsugamushi. They were tested for the detection of antibodies against O. tsutsugamushi in the patient's serum samples using enzyme-linked immunosorbent assay (ELISA) and dot-blot analyses. As of conventional immunofluorescence assay (IFA), when the mixture of these three recombinant antigens was used, both sensitivity and specificity of the recombinant antigens were increased up to 98% in IgM and IgG at ELISA and dot blotting. Additionally, both sensitivity and specificity by detection of IgM and IgG antibodies at rapid diagnostic test (RDT), using the mixture of three antigens and gold conjugated antibodies, were 99%. Our results suggest the use of mixture of these recombinant antigen proteins in ELISA or RDT is suitable as a diagnostic test for scrub typhus.
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KEYWORD
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Scrub Typhus, Orientia tsutsugamushi, Recombinant Antigen, IFA, ELISA, RDT
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